中文摘要：
為(wei)了(le)研(yan)(yan)究卵(luan)(luan)(luan)(luan)(luan)(luan)巢巨(ju)噬(shi)細(xi)胞(bao)(bao)(bao)群體(ti)在(zai)排(pai)(pai)卵(luan)(luan)(luan)(luan)(luan)(luan)中(zhong)的(de)(de)(de)(de)(de)作用，我們研(yan)(yan)究了(le)使(shi)用脂(zhi)(zhi)質(zhi)(zhi)(zhi)體(ti)封裝(zhuang)的(de)(de)(de)(de)(de)氯(lv)膦(lin)(lin)酸(suan)(suan)(suan)鹽(yan)消耗該群體(ti)的(de)(de)(de)(de)(de)影響(xiang)(xiang)。在(zai)排(pai)(pai)卵(luan)(luan)(luan)(luan)(luan)(luan)前 84 小(xiao)時(shi)(shi)（第 -3 天(tian)(tian)(tian)(tian)）或 36 小(xiao)時(shi)(shi)（第 -1 天(tian)(tian)(tian)(tian)）在(zai)促性(xing)(xing)腺激素(su)引發的(de)(de)(de)(de)(de)成年小(xiao)鼠的(de)(de)(de)(de)(de)卵(luan)(luan)(luan)(luan)(luan)(luan)巢滑囊下注射(she)氯(lv)膦(lin)(lin)酸(suan)(suan)(suan)脂(zhi)(zhi)質(zhi)(zhi)(zhi)體(ti)、鹽(yan)水(shui)(shui)(shui)脂(zhi)(zhi)質(zhi)(zhi)(zhi)體(ti)或單獨生理(li)鹽(yan)水(shui)(shui)(shui)。排(pai)(pai)卵(luan)(luan)(luan)(luan)(luan)(luan)率(lv)(lv)是通過計算釋(shi)放的(de)(de)(de)(de)(de)卵(luan)(luan)(luan)(luan)(luan)(luan)母細(xi)胞(bao)(bao)(bao)數(shu)(shu)量(liang)來(lai)(lai)確定(ding)的(de)(de)(de)(de)(de)。排(pai)(pai)卵(luan)(luan)(luan)(luan)(luan)(luan)前后也(ye)立即計算了(le)graafian卵(luan)(luan)(luan)(luan)(luan)(luan)泡和(he)(he)黃體(ti)的(de)(de)(de)(de)(de)數(shu)(shu)量(liang)。使(shi)用針對巨(ju)噬(shi)細(xi)胞(bao)(bao)(bao)抗(kang)原 macrosialin、主(zhu)要組(zu)織(zhi)相容性(xing)(xing)復合物 II 類 （Ia） 和(he)(he) F4/80 的(de)(de)(de)(de)(de)特(te)異(yi)性(xing)(xing)單克隆抗(kang)體(ti)進(jin)行免疫組(zu)化(hua)檢(jian)查排(pai)(pai)卵(luan)(luan)(luan)(luan)(luan)(luan)前卵(luan)(luan)(luan)(luan)(luan)(luan)巢的(de)(de)(de)(de)(de) theca 和(he)(he)基質(zhi)(zhi)(zhi)內(nei)的(de)(de)(de)(de)(de)巨(ju)噬(shi)細(xi)胞(bao)(bao)(bao)分布(bu)。在(zai)第 -1 天(tian)(tian)(tian)(tian)注射(she)氯(lv)膦(lin)(lin)酸(suan)(suan)(suan)脂(zhi)(zhi)質(zhi)(zhi)(zhi)體(ti)不影響(xiang)(xiang)排(pai)(pai)卵(luan)(luan)(luan)(luan)(luan)(luan)率(lv)(lv)，而在(zai)第 -3 天(tian)(tian)(tian)(tian)注射(she)氯(lv)膦(lin)(lin)酸(suan)(suan)(suan)脂(zhi)(zhi)質(zhi)(zhi)(zhi)體(ti)導(dao)致排(pai)(pai)卵(luan)(luan)(luan)(luan)(luan)(luan)率(lv)(lv)顯著降低（平均排(pai)(pai)卵(luan)(luan)(luan)(luan)(luan)(luan)卵(luan)(luan)(luan)(luan)(luan)(luan)母細(xi)胞(bao)(bao)(bao)分別為(wei) 5. 25 +/- 0.6 來(lai)(lai)自氯(lv)膦(lin)(lin)酸(suan)(suan)(suan)脂(zhi)(zhi)質(zhi)(zhi)(zhi)體(ti)處(chu)理(li)的(de)(de)(de)(de)(de)卵(luan)(luan)(luan)(luan)(luan)(luan)巢和(he)(he) 9.13 +/- 0.9 來(lai)(lai)自鹽(yan)水(shui)(shui)(shui)處(chu)理(li)的(de)(de)(de)(de)(de)卵(luan)(luan)(luan)(luan)(luan)(luan)巢，P < 0.05）。排(pai)(pai)卵(luan)(luan)(luan)(luan)(luan)(luan)時(shi)(shi) theca 中(zhong)存在(zai)的(de)(de)(de)(de)(de)巨(ju)唾(tuo)液酸(suan)(suan)(suan)陽性(xing)(xing)巨(ju)噬(shi)細(xi)胞(bao)(bao)(bao)的(de)(de)(de)(de)(de)數(shu)(shu)量(liang)通過第 -1 天(tian)(tian)(tian)(tian)的(de)(de)(de)(de)(de)氯(lv)膦(lin)(lin)酸(suan)(suan)(suan)脂(zhi)(zhi)質(zhi)(zhi)(zhi)體(ti)處(chu)理(li)減(jian)少，第 -3 天(tian)(tian)(tian)(tian)的(de)(de)(de)(de)(de)治療減(jian)少了(le) theca 中(zhong)存在(zai)的(de)(de)(de)(de)(de) Ia 陽性(xing)(xing)和(he)(he)巨(ju)唾(tuo)液素(su)陽性(xing)(xing)巨(ju)噬(shi)細(xi)胞(bao)(bao)(bao)的(de)(de)(de)(de)(de)數(shu)(shu)量(liang)。當通過陰(yin)道涂片檢(jian)查隨后的(de)(de)(de)(de)(de)卵(luan)(luan)(luan)(luan)(luan)(luan)巢周期(qi)時(shi)(shi)，發現氯(lv)膦(lin)(lin)酸(suan)(suan)(suan)脂(zhi)(zhi)質(zhi)(zhi)(zhi)體(ti)處(chu)理(li)的(de)(de)(de)(de)(de)動物的(de)(de)(de)(de)(de)中(zhong)胚層(ceng)-2/二元期(qi)延長（7.5 +/- 1.3天(tian)(tian)(tian)(tian)，鹽(yan)水(shui)(shui)(shui)脂(zhi)(zhi)質(zhi)(zhi)(zhi)體(ti)處(chu)理(li)動物為(wei)3.4 +/- 0.4天(tian)(tian)(tian)(tian)，P < 0.05）。這(zhe)些結果表明(ming)，鞘(qiao)狀巨(ju)噬(shi)細(xi)胞(bao)(bao)(bao)可(ke)能(neng)參與(yu)卵(luan)(luan)(luan)(luan)(luan)(luan)泡生長和(he)(he)破裂的(de)(de)(de)(de)(de)調節，并(bing)且(qie)對發情周期(qi)的(de)(de)(de)(de)(de)正常進(jin)展很重要。
英文摘(zhai)要：
To investigate the role of the ovarian macrophage population in ovulation, we examined the effect of depleting this population using liposome-encapsulated clodronate. Clodronate liposomes, saline liposomes, or saline alone was injected under the ovarian bursa in gonadotropin-primed adult mice, either 84 h (Day -3) or 36 h (Day -1) before ovulation. Ovulation rates were determined by counting the number of oocytes released. The numbers of graafian follicles and corpora lutea were also counted immediately before and after ovulation. Macrophage distribution within the theca and stroma of preovulatory ovaries was examined by immunohistochemistry with specific monoclonal antibodies to the macrophage antigens macrosialin, major histocompatability complex class II (Ia), and F4/80. Injection of clodronate liposomes on Day -1 did not affect ovulation rates, whereas administration on Day -3 caused a significant reduction in ovulation rate (mean oocytes ovulated = 5. 25 +/- 0.6 from clodronate liposome-treated ovaries and 9.13 +/- 0.9 from saline-treated ovaries, respectively, P < 0.05). The numbers of macrosialin-positive macrophages present in the theca at ovulation were reduced by treatment with clodronate liposomes on Day -1, and treatment on Day -3 reduced the numbers of Ia-positive and macrosialin-positive macrophages present in the theca. When the subsequent ovarian cycles were examined by vaginal smearing, the metestrous-2/diestrous stage was found to be extended in clodronate liposome-treated animals (7.5 +/- 1.3 days vs. 3.4 +/- 0.4 days for saline liposome-treated animals, P < 0.05). These results suggest that thecal macrophages may be involved in the regulation of follicular growth and rupture, as well as being important for the normal progression of the estrous cycle.
產品詳情：
品牌：LIPOSOMA
貨號：CP-005-005
產地：荷蘭
品(pin)名：Clodronateliposomes氯(lv)膦(lin)酸二(er)鈉脂質體巨噬細胞清除劑
實驗細節：
實驗結果：
論文題目：
該文章比較(jiao)久了，是較(jiao)早研(yan)究卵巢巨(ju)(ju)噬細胞(bao)(bao)的文章，可以借(jie)鑒(jian)。因為研(yan)究很早，所以數據很基(ji)礎(chu)。無論如(ru)何，我們(men)可以訂(ding)購荷(he)蘭liposoma品(pin)牌的巨(ju)(ju)噬細胞(bao)(bao)清(qing)除劑Clodronateliposomes，具體訂(ding)購聯系靶點科技。這(zhe)為研(yan)究清(qing)除卵巢巨(ju)(ju)噬細胞(bao)(bao)提供的研(yan)究基(ji)礎(chu)。
